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Current

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Duration:	1. 1. 2025 -
Evidence number:
Program:	Iné projekty
Project leader:	Mgr. Pavuková Eva, PhD.

Possibility of using intestinal in vitro and ex vivo models for studying the transport of manganese, intestinal functionality and epithelial integrity.
Duration:	1. 1. 2025 - 31. 12. 2028
Evidence number:	2/0036/25
Program:	VEGA
Project leader:	MVDr. Grešáková Ľubomíra, PhD.
SAS cosolvers:	RNDr. Takácsová Margaréta, PhD., Ing. Tokarčíková Katarína, PhD.
Annotation:	The use of intestinal in vitro and ex vivo models to simulate the digestive tract conditions is important for transportstudy, studying intestinal functionality and epithelial integrity. However, these models have not been used enoughfor clarifying the trace mineral transport yet. The project purpose is to investigate the transport of Mn, which is anessential as well as toxic trace element, playing a key role in the animal health and nutrition. Using the intestinalmodels, Ussing chambers system and primary intestinal epithelial cell cultures, Mn absorption will be investigatedto clarify its transport mechanisms, and the effect of Mn on the functions and properties of the gut epithelium. Theproject objective is to evaluate the suitability of the used intestinal models for studying the absorption andtransport, intestinal functionality and epithelial integrity, and optimising of cultivation and isolation of primaryintestinal epithelial cells, their characterization and cultivation as 2D and 3D cultures.

Postbiotics and their use for animal health
Duration:	1. 1. 2025 - 31. 12. 2028
Evidence number:	VEGA 2/0009/25
Program:	VEGA
Project leader:	MVDr. Pogány Simonová Monika, PhD
SAS cosolvers:	MVDr. Bino Eva, PhD., MVDr. Lauková Andrea, CSc., RNDr. Ščerbová Jana, PhD., MVDr. Zábolyová Natália
Annotation:	Methicillin-resistant (MetR) and vancomycin-resistant (VanR) staphylococci and enterococci occur in healthy farm animals and they can pose a risk to human health due to contamination of animal products. In addition, the migration of resistant microbiota into the environment causes an ecological burden and wildlife serves as a reservoir of resistant bacteria that are difficult to eliminate. The use of postbiotics – enterocins (antimicrobial proteinaceous substances produced by some strains of the genus Enterococcus) could be a promising way to eliminate MetR and VanR bacteria. Therefore, the present basic research project will focus on testing the inhibitory and antibiofilm activity of enterocins (characterized at our workplace) against a target of undesirable microbiota with an impact on MetR/VanR and biofilm-forming staphylococci and enterococci of animal origin. The results with enterocins achieved under in vitro condition against selected MetR/VanR strains will be verified using in vivo model experiments.

Progressive and new eco-friendly combating strategies against animal bacterial biofilm.
Duration:	1. 1. 2025 - 31. 12. 2028
Evidence number:	2/0040/25
Program:	VEGA
Project leader:	RNDr. Bujňáková Dobroslava, PhD.
SAS cosolvers:	Alexiová Zuzana, Mgr. Bombárová Alexandra, RNDr. Čobanová Klaudia, PhD., MVDr. Galambošiová Tímea, MVDr. Karahutová Lívia, PhD.
Annotation:	The main objective of the proposed project will be to obtain a new knowledge about the in vitro effect of variouseco-friendly game-changers leading to the modulation of bacterial biofilms in model species of food animalbacteria i.e. Gamma Proteobacteria (Escherichia, Klebsiella, Pseudomonas) and Staphylococcus. Research willbe focused on the molecular analysis of biofilm-associated bacteria (virulence and resistance-related genes).Selected substances will be applied to animal with aim to investigate their effect on intestinal microbiotacomposition and bacterial enzymatic activities.Anti-biofilm properties of selected non-antibiotic biological and chemical substances can help veterinarians in thefuture manage treatment of recurrent biofilm-associated infection and moreover progress in alternative strategiesmay help to combat (overcome) antibiotic resistance which is consistent with "Farm to Fork" European Unionstrategy plans to make the European food system fair, healthy, and more sustainable.

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Duration:	1. 1. 2025 - 31. 12. 2028
Evidence number:	2/0099/25
Program:	VEGA
Project leader:	RNDr. Kopčáková Anna, PhD.

Effect of low-frequency ultrasound in the treatment of wounds tested on a CAM model of Japanese quail
Duration:	1. 1. 2025 - 31. 12. 2028
Evidence number:	VEGA 2/0081/25
Program:	VEGA
Project leader:	Mgr. Máčajová Mariana, PhD.
SAS cosolvers:	RNDr. Bilčík Boris, PhD., MUDr. Čavarga Ivan, PhD., Mgr. Meta Majlinda
Annotation:	The treatment of infected wounds is one of the challenges in veterinary medicine. At the time of increasing antimicrobial resistance, other therapies are needed. Our project aims to develop in vivo model for research and treatment of infections. We will apply sonodynamic therapy using low-frequency ultrasound on a Japanese quail chorioallantoic membrane (CAM) model. Sonodynamic therapy is a therapeutic method that combines ultrasound and chemicals called sonosensitizers. Similar to photodynamic therapy (PDT), which uses light to activate photosensitizers, sonodynamic therapy uses for activation ultrasound. In the context of combination therapy, we also want to analyze other processes induced by low-frequency ultrasound, such as sonophoresis and hyperthermia. After therapy, we will monitor angiogenic changes in CAM tissue, expression of selected genes, as well as histological and immunohistochemical changes. The results obtained have potential applications in the treatment of wounds or superficial infections.

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Duration:	1. 1. 2025 - 31. 12. 2028
Evidence number:	2/0007/25
Program:	VEGA
Project leader:	Ing. Petrič Daniel, PhD.
SAS cosolvers:	RNDr. Batťányi Dominika, PhD., Mgr. Bombárová Alexandra, RNDr. Čobanová Klaudia, PhD., RNDr. Kišidayová Svetlana, CSc., MVDr. Leško Matej, MVDr. Váradyová Zora, PhD

IKPOSA - An innovative and comprehensive view of obstructive sleep apnea
Duration:	1. 9. 2024 - 30. 6. 2028
Evidence number:	APVV-23-0028
Program:	APVV
Project leader:	prof. Ing. Breier Albert, DrSc.
SAS cosolvers:	RNDr. Bertová Anna, PhD., Ing. Husieva Valeriia

KryoEmbryo - Cryotolerance of bovine embryos produced in vitro from cryopreserved oocytes
Duration:	1. 7. 2024 - 30. 6. 2028
Evidence number:	APVV-23-0203
Program:	APVV
Project leader:	RNDr. Antalíková Jana, PhD.
SAS cosolvers:	Ing. Horovská Ľubica, Ing. Jankovičová Jana, PhD., Ing. Michalková Katarína, PhD., Ing. Sečová Petra, PhD.
Other cosolvers:	RNDr. Elena Kubovičová, CSc., Ing. Lucia Olexíková, PhD., Ing. Linda Dujíčková, PhD.
Annotation:	An effective biotechnological method for increasing the utilization of the reproductive potential of cattle is the production of high-quality embryos in vitro. Cryopreservation of oocytes and embryos combined with in vitro fertilization (IVF) is an important tool for preserving genetic resources. In vitro production (IVP) of embryos from frozen oocytes can, in addition, significantly contribute to the expansion and preservation of the gene pool of endangered species or breeds of animals whose numbers are significantly decreasing. A problem with cryopreservation of IVP embryos is their poor resistance to freezing compared to in vivo embryos, which reduces the number of pregnancies in cows after embryo transfer. There are many indications that increasing the cryotolerance of pre-implantation embryos can be achieved by enriching culture media and vitrification solutions by adding some substances such as antioxidants, growth factors, cytoskeleton stabilizers and other additives that help to improve antioxidant protection, stabilize the cytoskeleton, affect lipid utilization and accumulation or other processes in cells. The presented project will be based on the results of our previous project, where we developed a system for successful cryopreservation of oocytes with a high post-warming survivability and maintenance of high developmental competence, thus, providing us with a rich source of cryopreserved bovine oocytes for further experiments. The project will be focused on increasing cryotolerance (resistance to cryopreservation) of embryos produced from vitrified oocytes by ensuring optimal culture conditions. New possibilities for the evaluation of cryotolerance of embryos will be defined based on the latest methods and techniques used in this area. The obtained results from the implementation of the project will be useful for the bank of animal genetic resources of male and female gametes and embryos.

MOMIKO - Microbiome modulators for maintaining dermal health in animals
Duration:	1. 7. 2024 - 30. 6. 2028
Evidence number:	APVV-23-0005
Program:	APVV
Project leader:	MVDr. Strompfová Viola, DrSc.
SAS cosolvers:	RNDr. Bujňáková Dobroslava, PhD., MVDr. Karahutová Lívia, PhD., MVDr. Štempelová Lucia, PhD.
Other cosolvers:	UVLF - Wolaschka Tomáš, Farbáková Jana
Annotation:	The skin - the largest organ of the body - harbors a wide variety of microbes that, together with their genetic information and host interactions, form the skin microbiome. Many of these microorganisms are harmless and in some cases even provide vital functions such as protection against invasion by the pathogenic organisms, modulation of the function of the skin\'s immune system, as well as acceleration of healing and reduction of inflammation in case of injury. Dysbiosis refers to the lack of balance among microbial communities within certain areas of the body that may lead to the onset or progression of diseases and is the focus of current scientific research. The goal is to find innovative methods to maintain the skin\'s microbial homeostasis and to avoid the use of antimicrobial treatment, which except the eradication of pathogens causing skin disease, also reduces beneficial part of the microbiome. Therefore, the intention of the project is to find an effective alternative means to strictly antibacterial agents in the form of an antibiotic-sensitive, safe and effective bacterium selected from the commensal skin microbiota of healthy animals, which can be applied to skin lesions to promote healing during dermatological diseases complicated by infection. Such an approach to therapy prevents further spread of resistance to antibiotics and thus the ineffectiveness of drugs due to resistance and helps to eliminate the use of non-ecological drugs.

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Duration:	1. 1. 2024 - 31. 12. 2027
Evidence number:	2/0045/24
Program:	VEGA
Project leader:	MUDr. Grinchii Daniil, PhD.

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Duration:	1. 1. 2024 - 31. 12. 2027
Evidence number:	1/0157/24
Program:	VEGA
Project leader:	Ing. Poturnayová Alexandra, PhD.
SAS cosolvers:	Ing. Domšicová Michaela, PhD., Mgr. Korčeková Jana, PhD., Mgr. Macková Katarína, PhD.
Annotation:	The aim of the project is to develop and validate a biosensor for microRNA detection for early diagnosis ofhaemato-oncological diseases. MiRNAs are known for their unique expression profiles and are consideredpromising biomarkers for early detection of several types of cancer. The proposed biosensors will be based onspecific oligonucleotide aptamers which provide advantages: high selectivity, affinity, stability. The project willinvolve several phases including design and selection of aptamers, optimisation of biosensors and evaluation oftheir performance in patient samples. The biosensors will be fabricated by immobilizing the aptamers on solid Ausurfaces or modified Au nanoparticle surfaces. Performance will be evaluated in terms of sensitivity, specificity,selectivity and reproducibility with limits of detection and linear ranges compared to conventional methods. Thesuccessful development of aptasensors could revolutionise cancer diagnostics by enabling early and effectivetherapeutic interventions.

Phosphorylation of the ryanodine receptor/Ca2+ channel as an effective regulator of inter-channel communication
Duration:	1. 1. 2024 - 31. 12. 2027
Evidence number:	2/0010/24
Program:	VEGA
Project leader:	Mgr. Gaburjáková Marta, PhD.
SAS cosolvers:	Mgr. Gaburjáková Jana, PhD., MUDr. Paliokha Ruslan
Annotation:	The ryanodine receptor (RyR)/Ca2+ channel is one of critical components implicated in muscle and neuronalCa2+ signaling. In vivo and in vitro, RyR channels self-organize into larger clusters creating highly favorableconditions for direct inter-channel communication. Molecular mechanisms of this phenomenon, which ismanifested by synchronized (coupled) gating of numerous RyR channels, are not understood at all. The projectaim is to show that in vitro induced phosphorylation of RyR channels substantially affects the appearance andstability of coupled gating in the heart. Furthermore, we are interested in the effect of chronic in vivophosphorylation of RyR channels, which has been evidenced in various pathological conditions of the heart andbrain. We assume that stabilization of coupled gating might result in significant advancements in the treatment ofchannelopathies related to RyR dysfunction; therefore, the important part of the project will be testing the effect ofa new promising drug, Rycal-S107.

Characterization of the equine skin microbiome and its targeted modulation
Duration:	1. 1. 2024 - 31. 12. 2027
Evidence number:	2/0004/24
Program:	VEGA
Project leader:	MVDr. Strompfová Viola, DrSc.
SAS cosolvers:	MVDr. Baran Vladimír, CSc., MVDr. Štempelová Lucia, PhD.
Annotation:	he skin, the largest and most exposed organ by surface area, represents an ecological niche for an enormous spectrum of microorganisms that contribute to the barrier function of the skin and skin homeostasis. Any changes in the population of commensal microorganisms are associated with physiological changes occuring during various dermatological diseases or aging. Nevertheless, the amount of knowledge on the composition, diversity and functions of the skin microbiota in horses is negligible. Knowing the taxonomic composition of the microbiota under physiological and pathological conditions and its properties, what is the goal of this project, will help with the search for appropriate therapeutic strategies in relatively frequently occurring dermatological diseases in horses, will help to improve welfare by accelerating healing, and will help to reduce the danger of antibiotic-resistant bacterial strains. The project will study also antimicrobial effect of bioactive substances for reduction of skin infection.

Novel approaches for effective utilization of phytoadditives in animal nutrition.
Duration:	1. 1. 2024 - 31. 12. 2027
Evidence number:	VEGA 2/0005/24
Program:	VEGA
Project leader:	MVDr. Plachá Iveta, PhD
SAS cosolvers:	RNDr. Bačová Kristína, PhD., prof. MVDr. Faix Štefan, DrSc., RNDr. Kopčáková Anna, PhD., Mgr. Krištofová Radoslava, RNDr. Takácsová Margaréta, PhD.
Annotation:	The concept of „gut health“ has recieved a lot of attention as it has been recognized as one of the key elements in determining animal performance. Even though, plant phenolic compounds are able to improve gut health due to their strong antioxidant potential, further studies are needed to understand how the processes of their biotransformation in the gastrointestinal tract can affect their bioavailability in organism. One of the crucial aspects of their beneficial effect are the amount present in the gut as a result of their release from feed, and their consequent ability to pass through the intestinal wall. Before application to animals, more studies are urgently needed to precisely understand the metabolic processes of plant compounds within organisms. The main idea of project proposal are biotransformation processes of stabilised form of thymol, main compound of thyme, in rabbit´s organism which represents an appropriate animal model for evaluation of the bioavailability of nutrients.

Causes and consequences of birdsong variability
Duration:	1. 1. 2024 - 31. 12. 2027
Evidence number:	2/0150/24
Program:	VEGA
Project leader:	Mgr. Niederová-Kubíková Ľubica, PhD.
SAS cosolvers:	Mgr. Hoďová Vladimíra, RNDr. Košťál Ľubor, CSc., Mgr. Pavuková Eva, PhD., Mgr. Radič Rebecca
Annotation:	The ability of songbirds to imitate conspecific sounds shares similarities with human speech. Species vary in boththe size of their repertoire and the variability of their songs. The aim of this project is to investigate the causes ofthe variability and its significance. As higher song variability is associated with higher neurogenesis, we proposeto investigate whether the elimination of new neurons leads to reduced variability. We will use anadeno-associated virus to reduce the recruitment of new neurons in vocal regions controlling birdsong andquantify the viral effects on neurogenesis and songs. We will study the significance of song variability innon-singing females that learn and recognize songs from different males. We will determine whether they canrecognize songs differing in variability. The results of this project will show whether the function of neurogenesisin the adult songbirds is to introduce variability into song and whether this variability is of practical importance inmate choice.

Study of processes associated with bull sperm maturation
Duration:	1. 1. 2024 - 31. 12. 2027
Evidence number:	2/0074/24
Program:	VEGA
Project leader:	Ing. Jankovičová Jana, PhD.
SAS cosolvers:	RNDr. Antalíková Jana, PhD., Ing. Horovská Ľubica, Ing. Michalková Katarína, PhD., Ing. Sečová Petra, PhD.
Annotation:	In mammals, fertilization can be characterized as a complex process, a sequence of several highly ordered events. The prerequisite for gamete fusion is their morphological and functional maturity. The project aims to study the molecules involved in the maturation of male gametes, i.e. the processes from sperm development in the process of spermatogenesis in the testis, sperm maturation in the epididymis to ejaculation. The aim of the project is to analyse proteins and lipids in spermatozoa at different stages of maturity, somatic cells of male reproductive organs and extracellular vesicles, and we will study their glycosylation modifications, distribution and dynamics using the bovine as a model organism. With the intention of studying the process of sperm capacitation, we will attempt to develop an optimal method to detect the capacitation status of bovine spermatozoa. The results obtained may contribute to understanding the physiology of mammalian reproduction as a whole under physiological and pathological conditions.

DNA methylation changes accompanying the development of multidrug resistance
Duration:	1. 1. 2024 - 31. 12. 2027
Evidence number:	2/0046/24
Program:	VEGA
Project leader:	Mgr. Pavlíková Lucia, PhD.
SAS cosolvers:	RNDr. Boháčová Viera, CSc., Ing. Husieva Valeriia, RNDr. Kšiňanová Martina, PhD., Ing. Pelegrinová Lívia, Ing. Sulová Zdena, DrSc., Mgr. Šereš Mário, PhD., Ing. Šimoničová Kristína, PhD.
Annotation:	The development of multidrug resistance (MDR), mediated by the overexpression of ABC transporters, represents a real obstacle to overcome in cancer chemotherapy. The MDR phenotype is also accompanied by the activation of other defense mechanisms that protect the cell from damage by cytostatics, including those that are not substrates of ABC transporters. These mechanisms may be of dual origin. They may be directly related to the presence of ABC transporters in the cells due to common mechanisms controlling their induction, or they may have developed due to the selection pressure of cystostatics during treatment but independently of the induction of ABC transporter expression. Alterations in the methylation of promoter regions of genes, the modulation of which may be involved in the emergence of multidrug resistance, may be responsible for the wide variability in cellular responses to cytostatic-induced chemical stress and the frequently observed changes in the state of cell differentiation.

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Duration:	1. 9. 2023 - 31. 8. 2027
Evidence number:	09I03-03-V02-00020
Program:	Plán obnovy EÚ
Project leader:	Ing. Husieva Valeriia

Lipid metabolism and its role in mitochondrial bioenergetics
Duration:	1. 9. 2023 - 31. 8. 2027
Evidence number:	09I03-03-V02-00020
Program:	Plán obnovy EÚ
Project leader:	Mgr. Zriniová Ingrid

BPKMKZHPMF - Non-antibiotic approaches to control mastitis of cows with an increase in the hygiene of dairy farms conditions
Duration:	1. 7. 2023 - 30. 6. 2027
Evidence number:	APVV-22-0457
Program:	APVV
Project leader:	RNDr. Bujňáková Dobroslava, PhD.
SAS cosolvers:	Augustinská Danuše, MVDr. Galambošiová Tímea, MVDr. Karahutová Lívia, PhD., Timková Iveta
Annotation:	Inflammation of the mammary gland ‒ mastitis is currently one of the three main diseases encountered by dairyfarmers. The most common treatment of mastitic cows is antibiotic therapy during lactation, but also all rounddrying programmes. However, frequent use of antibiotics results in several serious problems such as lowtreatment success, and the presence of antimicrobial residues in milk. For this reason, the EU by its "Farm to Fork"strategy plans to reduce the use of antibiotics for farm animals by 50%, which is also supported by a new laweffective from January 2022, that prohibits all forms of routine use of antibiotics and zinc oxide, includingpreventative herd treatment and all round drying of cows. In Slovakia as well as in the neighboring EU countries, nomethodological procedures have been established for dairy farmers regarding the drying of cows in order to reducethe use of antibiotics. The presented project comprehensively resolves the current problems encountered by dairyfarmers focusing on practical rationalization and implementation of new procedures for diagnosis, suppression andprevention of mastitis. The introduction of new diagnostic methods for the direct and indirect determination of inflammation symptoms supplemented by analysis of dairy cows based on clinical examination and the results ofmilk utility control with the application of algorithmic models for the detection of risky cows are among the basicobjectives of the project aimed at reducing the incidence of mastitis. One of the new components of the project tobe implemented in practice in order to reduce the consumption of antibiotics will include the introduction ofselective drying programmes for dairy cows and the development of an effective lactobacilli-based protective agentfor udder treatment. A new disinfectant for complex elimination of pathogens from the environment of the milkingparlor will be designed by the research team for use in practice conditions.

FATSPOMBE - Functional analyses of TOR signaling pathway in the regulation of abiotic stress response in the fission yeast
Duration:	1. 7. 2023 - 30. 6. 2027
Evidence number:	APVV-22-0294
Program:	APVV
Project leader:	Mgr. Bágeľová Poláková Silvia, PhD.
SAS cosolvers:	Mgr. Bakošová Anetta, PhD.
Annotation:	As a consequence of our modern lifestyle, each organism is at the daily basis attacked by various impairing agents, oxidants, inducing elevation of reactive oxygen species (ROS) by individual cells. Understanding the influence of those toxic elements on biochemical and physiological processes considerably contributes to specification of their toxicity. TOR kinase, a highly conserved kinase between species, belongs to the main regulators of cell growth and metabolism in response to various stresses. The aim of the proposed project is to investigate the molecular mechanism of TOR signaling in the cell defense machineries and cell cycle regulation under food toxicants acrylamide and its metabolites-induced stress, with the use of Schizosaccharomyces pombe as model system. S. pombe is a popular model organism for studies of basic biological processes. It is often referred to as “micromammal” as its genomic organization resembles that of higher eukaryotes including humans. Similar to higher eukaryotes, TORC1 and TORC2 have been identified in S. pombe. S. pombe TORC1, which contains the catalytic subunit Tor2, and TORC2, which contains Tor1 apparently have reverse, but coordinated, roles for growth, cell cycle and sexual development. Although, the role of TOR signaling in cell responses to various stresses has been intensively studied, the underlying mechanism of TORC2 and its catalytic subunit Tor1 in S. pombe in the cell protection and cell cycle regulation under stress conditions caused by food contaminants remains unclear. We aim to utilize state-of-the art research “OMIC” methods in molecular biology, genetics, spectroscopy, biochemistry, microscopy, and visualization to achieve the proposed objectives.

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Duration:	1. 7. 2023 - 30. 6. 2027
Evidence number:	APVV-22-0061
Program:	APVV
Project leader:	Mgr. Gaburjáková Marta, PhD.
SAS cosolvers:	MSc. Adnan Mohammad, Mgr. Bukatová Stanislava, PhD. , RNDr. Cagalinec Michal, PhD., MMedSc Dremencov Eliyahu, DrSc, Mgr. Gaburjáková Jana, PhD., MUDr. Grinchii Daniil, PhD., RNDr. Krejčíová Eva, RNDr. Mach Mojmír, PhD., RNDr. Májeková Magdaléna, PhD., Mgr. Novak Anastasiia, MUDr. Paliokha Ruslan, RNDr. Zahradníková, ml. Alexandra, PhD., Mgr. Zvozilová Alexandra

mClicID - -
Duration:	1. 7. 2023 - 30. 6. 2027
Evidence number:	APVV-22-0085
Program:	APVV
Project leader:	Mgr. Ševčíková Tomášková Zuzana, PhD.
SAS cosolvers:	Mgr. Andelová Natália, PhD., Ing. Ferko Miroslav, PhD., Mgr. Grman Marián, PhD., RNDr. Ondáčová Katarína, PhD., Mgr. Polčicová Katarína, PhD., Ing. Poturnayová Alexandra, PhD.
Annotation:	Mitochondrial chloride channels are involved in the regulation of the mitochondrial membrane potential Ψm. In in vitro conditions, it was observed that oxidative stress results in oscillations of Ψm, which leads to the shortening of the action potential on the plasma membrane of cardiomyocytes and the occurrence of arrhythmias, mediated by the production of ATP in the mitochondria. At the level of the whole heart, arrhythmias were observed as a consequence of ischemia-reperfusion. Specific ligands of the translocator protein (TSPO) prevent the occurrence of post-ischemic arrhythmias. The use of a non-specific chloride channel blocker led to the same effect. TSPO ligands inhibit the mitochondrial chloride channels at nanomolar concentrations, suggesting that the TSPO protein mediates channel block. Thus, TSPO is likely to be in close contact with the chloride channel. Mitochondrial chloride channels are well described at the electrophysiological level, but their molecular identity remains unclear. Recently, two isoforms of chloride intracellular channels (CLICs) have been shown to be localized in mitochondria. However, CLIC channels have only been described in an artificial system - overexpressed in host cells. Mitochondrial chloride channels from native membranes are assumed to be identical to one of the two mitochondrial CLIC isoform. The aim of the presented project is to verify the hypothesis that the measured native chloride channels from cardiac mitochondria are members of the CLIC family and whether the given CLIC isoform and TSPO are in close physical contact. We assume that the obtained results will help clarify the molecular identity of the mitochondrial chloride channel, which represents a significant potential target for preventing the occurrence of post-ischemic arrhythmias.

PUFA-REPRO-BOOST - Prevention of periconceptional reproductive failure in animals via supplementation of diet with various sources of polyunsaturated fatty acids
Duration:	1. 7. 2023 - 30. 6. 2027
Evidence number:	APVV-22-0071
Program:	APVV
Project leader:	MVDr. Fabian Dušan, DrSc.
SAS cosolvers:	RNDr. Babeľová Janka, PhD., RNDr. Čikoš Štefan, DrSc., RNDr. Kovaříková Veronika, PhD., PhDr. Pisko Jozef, PhD., Mgr. Rušinová Laura, MVDr. Šefčíková Zuzana, CSc., RNDr. Špirková Alexandra, PhD.
Other cosolvers:	Univerzita veterinárskeho lekárstva a farmácie v Košiciach
Annotation:	In recent decades, many studies have reported a worrisome decrease in the reproductive performance of farm animals. Dietary lipid supplementation can be a good strategy to reduce the extent of reproductive failure. Oilseed meals and cakes, derived from seed oil extraction, represent interesting co-products usable for such purposes. The main aim of the project will be to evaluate the effect of diet supplementation with flaxseed and camelina cake, important sources of polyunsaturated fatty acids (PUFAs), on the ability to conceive and to reduce early embryo loss in two model animals - pubertal gilts and mouse females. Experimental animals will be fed a PUFAs supplemented diet for several weeks during the preconception period. After assessment of basic reproductive parameters, biochemical alterations in the microenvironment of developing germ cells and the impact of such alterations on antioxidant activity and membrane integrity of oocytes and proliferative and reparative activity of preimlantation embryos will be evaluated. To investigate the mechanisms underlying the impact of diet supplementation, expression of relevant molecules (receptors for hormones, prostaglandins, biosynthetic enzymes, regulatory proteins, etc.) in oocytes, embryos and tissues of the reproductive tract will be assessed. Furthermore, the protective potential of diet supplementation by PUFAs in reproductive disorders related to aging and oxidative stress in vitro will be assessed. Study will bring novel information on the mechanisms underlying the impact of PUFAs on ovulation and early embryonic development. Furthemore, acquired knowledge would result in targeted use in livestock production and veterinary practice.

RETATGZPP - Use of recombinant enzymes with thioglucosidase activity for the transformation of plant glucosinolates and their analogues into biologically active substances with preventive and suppressive effect on neoplasia development
Duration:	1. 7. 2023 - 30. 6. 2027
Evidence number:	APVV-22-0383
Program:	APVV
Project leader:	RNDr. Imrichová Denisa, PhD.
SAS cosolvers:	RNDr. Bertová Anna, PhD., Ing. Kontár Szilvia, PhD., Ing. Sulová Zdena, DrSc., Ing. Šimoničová Kristína, PhD., Yoldi Vergara Alberto , MSc.

Identification of behavioural and neurobiological indicators of positive poultry welfare
Duration:	1. 1. 2023 - 31. 12. 2026
Evidence number:	2/0129/23
Program:	VEGA
Project leader:	RNDr. Košťál Ľubor, CSc.
SAS cosolvers:	Mgr. Pichová Katarína, PhD.
Annotation:	In the field of animal welfare science, there is a marked shift from negative welfare, the study of phenomena suchas fear, pain or suffering, to positive welfare. In addition to looking for indicators of positive welfare, this alsomeans looking for ways how to induce positive affective states, positive welfare. The aim of the project is to testthe possibility of inducing positive welfare in poultry by anticipation of reward, positive contrast in the magnitudeof reward or positive control of animals over their environment or resources. We will test the possibilities ofautomating behavioural recording as an important indicator of welfare and the hypothesis that the intensity ofneurogenesis in the hippocampus may serve as a marker of cumulative affective experience.

Infrared thermography as a tool for the study of emotions and animal welfare
Duration:	1. 1. 2023 - 31. 12. 2026
Evidence number:	2/0105/23
Program:	VEGA
Project leader:	Mgr. Pichová Katarína, PhD.
SAS cosolvers:	RNDr. Bilčík Boris, PhD., RNDr. Košťál Ľubor, CSc.
Annotation:	Thanks to the massive development of new technologies in recent years these have also become a part ofagricultural production. Precision Livestock Farming (PLF) represents a new system of animal farm productionwhich applies technologies to monitor the health and welfare of animals to improve the production and quality oflife of the animals. Infrared thermography, due to its non-invasiveness, represents a potential tool to measure thewelfare of poultry from several perspectives, whether assessing external indicators such as the degree ofplumage damage, but also physiological parameters detecting affective states through changes in temperature ofperipheral body regions. The aim of the project is to optimize the use of infrared thermography to assess welfarein laying hens, to test its application in commercial breeding conditions, and thus contribute to the development ofparameters objectively assessing the quality of life of animals.

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Duration:	1. 1. 2023 - 31. 12. 2026
Evidence number:	2/0051/23
Program:	VEGA
Project leader:	Mgr. Ševčíková Tomášková Zuzana, PhD.
SAS cosolvers:	RNDr. Ondáčová Katarína, PhD.
Annotation:	Cardiac arrhythmias are one of the most common causes of death in the world. Arrhythmias also arise as a result of ischemia/reperfusion, when changes in nutrient and oxygen supply occur. Oxygen radicals may increase during reperfusion. Both metabolic and oxidative stress have been shown to result in arrhythmias due to cyclic changes in the mitochondrial membrane potential. These changes are mediated by chloride channels, which are thought to be identical to the CLIC5 isoform of intracellular chloride channels. Cardioprotective effects at the level of cells and the whole heart were observed after application of a specific ligand of mitochondrial translocator protein TSPO - 4-chlorodiazepam (4Cl-DZP), but also after application of a non-specific anion channel inhibitor. 4Cl-DZP is thought to act on chloride channel activity indirectly, via the TSPO protein, but no details of this interaction are known. The aim of our project is to describe the relationship between CLIC5 chloride channels and the TSPO receptor.

Molecular mechanisms of preimplantation embryo responses to environmental factors
Duration:	1. 1. 2023 - 31. 12. 2026
Evidence number:	2/0041/23
Program:	VEGA
Project leader:	RNDr. Čikoš Štefan, DrSc.
SAS cosolvers:	RNDr. Babeľová Janka, PhD., MVDr. Fabian Dušan, DrSc., RNDr. Kovaříková Veronika, PhD., PhDr. Pisko Jozef, PhD., Mgr. Rušinová Laura, MVDr. Šefčíková Zuzana, CSc., RNDr. Špirková Alexandra, PhD.
Annotation:	The project deals with the preimplantation embryo development, focusing on the mechanisms by which the early embryo responds to environmental factors. The role of selected cellular receptors will be investigated with emphasis on possible interactions between receptors activated by the same or by concurrent ligands (glutamate, glycine, GABA, selected insecticides). The impact of receptor ligand transporters will also be investigated. Selected intracellular signaling molecules and processes involved in the activation of the identified receptors as well as possible alternative mechanisms (such as oxidative stress) activated by the action of the investigated ligands will be analyzed. Understanding the mechanisms by which environmental factors affect the development of the preimplantation embryo can contribute to the development of husbandry, nutrition and animal health management practices that lead to a reduction of economic losses in livestock production caused by earlyembryo loss or later health disorders.

Lipid transfer proteins – study of molecular mechanisms and their potential as targets in treatment of selected human diseases.
Duration:	1. 1. 2023 - 31. 12. 2026
Evidence number:	2/0047/23
Program:	VEGA
Project leader:	RNDr. Griač Peter, DrSc.
SAS cosolvers:	Ing. Pokorná Lucia, Šťastný Dominik, M.Sc., RNDr. Tahotná Dana, CSc.
Annotation:	The aim of the project is to investigate the molecular mechanisms of selected lipid-transfer proteins and thepossibilities of their use as targets for the treatment of human diseases caused by microorganisms and parasites.We will focus on two types of diseases: those caused by yeast and fungi, and malaria caused by the protozoanPlasmodium falciparum. The absence of phosphatidylinositol transfer protein (PITP) Pdr16 causes an increase inthe susceptibility of yeast microorganisms to clinically used antifungals. To understand why, we will investigate ina model organism, the yeast Saccharomyces cerevisiae, the physiological role and molecular mechanism ofaction of the Pdr16 protein and its closely related Pdr17 protein. In P. falciparum, we will study two PITPs with theCRAL-TRIO structural domain that these proteins share with S. cerevisiae Sec14p. We will take advantage of theknowledge that Sec14p can be inhibited by molecules, some of which are under clinical investigation as potentialantifungals.

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Duration:	1. 1. 2023 - 31. 12. 2026
Evidence number:	2/0030/23
Program:	VEGA
Project leader:	prof. Ing. Breier Albert, DrSc.
SAS cosolvers:	RNDr. Bertová Anna, PhD., RNDr. Boháčová Viera, CSc., RNDr. Imrichová Denisa, PhD., Ing. Kontár Szilvia, PhD., RNDr. Kšiňanová Martina, PhD., Mgr. Pavlíková Lucia, PhD., Ing. Pelegrinová Lívia, Ing. Sulová Zdena, DrSc.

Elucidation of the function of selected genes in meiotic division in the yeast Schizosaccharomyces pombe
Duration:	1. 1. 2023 - 30. 12. 2026
Evidence number:	1/0340/23
Program:	VEGA
Project leader:	Mgr. Bágeľová Poláková Silvia, PhD.
SAS cosolvers:	Mgr. Bakošová Anetta, PhD.
Other cosolvers:	Prof.

CogEnt - Automated learning device as a cognitive enrichment for laying hens
Duration:	1. 10. 2024 - 30. 6. 2026
Evidence number:	09I03-03-V04-00374
Program:	Plán obnovy EÚ
Project leader:	Mgr. Pichová Katarína, PhD.

Characterization of excitatory and inhibitory neurons in the brain areas relevant for development of social behaviour in the autismrelated model
Duration:	1. 7. 2022 - 30. 6. 2026
Evidence number:	APVV-21-0189
Program:	APVV
Project leader:	Mgr. Jurkovičová Tarabová Bohumila, PhD.
SAS cosolvers:	Ing. Dubiel Lucia, PhD., prof. RNDr. Lacinová Ľubica, DrSc., Mgr. Ševčíková Tomášková Zuzana, PhD.

MABBIOM/MABBE - Modern approaches of the bacterial biofilms modulation (eradication)
Duration:	1. 10. 2024 - 30. 6. 2026
Evidence number:	09I03-03-V04-00364
Program:	Plán obnovy EÚ
Project leader:	MVDr. Karahutová Lívia, PhD.

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Duration:	1. 7. 2022 - 30. 6. 2026
Evidence number:	APVV-21-0473
Program:	APVV
Project leader:	Mgr. Gaburjáková Marta, PhD.
SAS cosolvers:	Mgr. Gaburjáková Jana, PhD.

Nerves to heart: genetic cardiovascular diseases influenced by neuronal dysfunction on the model of dilated cardiomyopathy with Lamin A/C mutation
Duration:	1. 10. 2024 - 30. 6. 2026
Evidence number:	09I03-03-V04-00478
Program:	Plán obnovy EÚ
Project leader:	Mgr. Macková Katarína, PhD.

ALTZINC - Potential and prospect of alternative zinc sources in animal nutrition
Duration:	1. 7. 2022 - 30. 6. 2026
Evidence number:	APVV-21-0301
Program:	APVV
Project leader:	RNDr. Čobanová Klaudia, PhD.
SAS cosolvers:	RNDr. Batťányi Dominika, PhD., Mgr. Bombárová Alexandra, RNDr. Bujňáková Dobroslava, PhD., MVDr. Demčáková Klára, prof. MVDr. Faix Štefan, DrSc., MVDr. Galambošiová Tímea, MVDr. Grešáková Ľubomíra, PhD., Ing. Petrič Daniel, PhD., RNDr. Takácsová Margaréta, PhD., MVDr. Váradyová Zora, PhD
Annotation:	The presented project proposal is focused on the use of various zinc nanoparticles and organic zinc sources as mineral supplements in animal nutrition and their effect on microelements absorption, retention, and utilisation in ruminants and poultry. Investigation of the effects of various dietary zinc sources on physiological processes and microbial parameters of the gastrointestinal tract of ruminants and poultry should bring new principal results. In general, the experiments will be aimed at improving the mineral status and optimising digestive processes in farm animals in an effort to reduce zinc excretion and thus environmental pollution of minerals from animal production. Research on the distribution of specific zinc-containing metalloproteins in animal tissues and their dependence on appropriate delivery of zinc in the form with high bioavailability may provide us with new basic information which in turn may be useful in practice for better prevention of oxidative stress and its negative consequences. The impact of various zinc nanoparticles in the animal feed on the quality of products of animal origin will be investigated as well. The basic knowledge obtained by fulfilling of objectives of the proposed project should also lay the foundation for future applied research on the supplementation of feeds with bioavailable zinc sources in order to improve animal health and performance.

DRPGE - The role of DNA repair proteins in gene repression
Duration:	1. 7. 2022 - 30. 6. 2026
Evidence number:	APVV-21-0210
Program:	APVV
Project leader:	Mgr. Bágeľová Poláková Silvia, PhD.
SAS cosolvers:	Mgr. Bakošová Anetta, PhD., Mgr. Balážová Mária, PhD., Ing. Čipák Ľuboš, PhD., Ing. Čipáková Ingrid, PhD., Mgr. Holič Roman, PhD., Mgr. Valachovič Martin, PhD.
Annotation:	Eukaryotic cells have evolved two mechanistically distinct pathways to repair double-strand breaks during mitosis: non-homologous end joining (NHEJ) and homologous recombination (HR). More recently, HR proteins have been shown to play critical roles in maintaining genome integrity during DNA replication. Additionally, our latest research showed that Dbl2 protein along with other HR proteins act in concert with HIRA histone chaperone to assemble repressive chromatin near hard-to-replicate sites. Notably, cells lacking either HR proteins or HIRA factors exhibited increased levels of antisense RNA and RNA from the LTR retrotransposons and subtelomeric genes. Furthermore, the localization of the misregulated genes significantly correlated with repetitive DNA elements such as LTRs. However, the mechanism underlying this phenomenon remains largely unknown. In this project, we propose that the observed repression of gene expression is an integral part of the recombination-dependent replication pathway. We postulate that Sap1 binding to LTRs blocks replication fork progression and results in stalled or collapsed replication forks. Collapsed replication forks are remodeled and relocated to nuclear periphery. The relocation of damaged DNA to nuclear periphery can provide an access to a set of factors necessary for particular pathways of DNA repair. At nuclear periphery both imprecise NHEJ and break-induced replication pathways are favored. After the repair, the affected hard-to-replicate sites might be quickly silenced by factors such as HIRA to avoid a toxic gene expression. In this project, we intend to use classical genetics, high-resolution microscopy and advanced methods based on whole genome sequencing such as MNase-seq and ChIP-seq to obtain a more quantitative picture of chromatin dynamics. We believe that the findings of this research will have broad implications to the field of cancer research and cell division.

COMPNEURO-ED - Computational Modeling of Neurons: HCN and Calcium Channels in Excitability & Depression
Duration:	1. 10. 2024 - 30. 6. 2026
Evidence number:	09I03-03-V04-00388
Program:	Plán obnovy EÚ
Project leader:	Mgr. Tomko Matúš, PhD.
Annotation:	Mental disorders, including depression, are serious public health problems. Particular attention needs to be paid to women during pregnancy, when hormonal fluctuations make women more vulnerable to stress and mood disorders. The negative effects of maternal depression on the offspring have been described on a number of different levels, ranging from changes in behaviour to anatomical changes in individual structures of the brain. Particular attention has been paid to the hippocampus, the centre of memory and cognition. Along with the prefrontal cortex, the hippocampus is vulnerable to stress. Changes in the excitability of hippocampal neurons of offspring as a result of maternal depression have also been observed in the Department of Biophysics and Electrophysiology at the Centre of Biosciences. However, there is still a lack of knowledge about the molecular determinants and signalling pathways underlying these changes. The aim of this project is to explain the observed changes in neuronal excitability by changes at the level of ion channels, specifically HCN and voltage-gated calcium channels, using computational modelling techniques. In addition, we will investigate whether maternal depression can affect key functions of hippocampal neurons such as resonance, temporal coding or coincidence detection. The data and models will be integrated into the EBRAINS, European research infrastructure. This will ensure their accessibility and potential use by other researchers. The project is also in line with the 3Rs principles of reduction of animal testing. By combining computer modelling, multi-objective optimisation techniques and statistical methods, the project will go beyond the current state of the art. Instead of an average model, a whole population of neurons will be created and studied to cover biological variability. Furthermore, the project will generate testable predictions.

Analysis of staphylococci isolated from skin of horses
Duration:	1. 7. 2024 - 31. 12. 2025
Evidence number:	APD0011
Program:	PostdokGrant
Project leader:	MVDr. Štempelová Lucia, PhD.
Annotation:	Currently, horses are used in the cultural and sports field, forestry, police, but also in the treatment of people in thefield of hippotherapy. Bacterial skin infections are important breeding and economic problems in horses. For thisreason, the aim of this project is the isolation and characterization of the skin microbiota in horses with a focus on thegenus Staphylococcus. Since staphylococci as opportunistic pathogens are often involved in the development ofdermatological diseases, the aim of this project will be to analyse the characteristics of collected staphylococci.Mapping resistance to antibiotics will provide important information in determining priorities in solving the currentantibiotic crisis. Testing other properties such as biofilm formation, toxins or other virulence factors will reveal theextent to which staphylococci can participate in pathological processes in the case of skin infections in horses orserve as a reservoir of genes for their transfer. Thorough knowledge of the microbial population is the basis for thedevelopment of new therapeutic approaches without the use of antibiotics.

Biotechnological potential of nonconventional yeast in the production of squalene
Duration:	1. 1. 2022 - 31. 12. 2025
Evidence number:	2/0036/22
Program:	VEGA
Project leader:	Mgr. Garaiová Martina, PhD.
SAS cosolvers:	Mgr. Holič Roman, PhD., Mgr. Krajčiová Daniela
Annotation:	Ergosterol biogenesis in yeast has been studied so far mainly in Saccharomyces cerevisiae traditionally used inbasic research and industry. It is quite surprising that very little is known about sterol homeostasis even in thelarge group of so-called nonconventional oleagionus yeasts utilized as producing organisms for various valuablelipids. The project is aimed at the study of sterol homeostasis in oleaginous yeasts Yarrowia lipolytica andRhodosporidium toruloides and on the role of lipid droplets in this process. Using the methods of metabolicengineering we will evaluate the potential of these yeasts in production of squalene, a precursor in ergosterolbiosynthesis with applications in cosmetic and pharmaceutic industry. We will also try to identify othernonconventional yeast species able to accumulate high amounts of squalene. The results of the project shouldcontribute to the extension of the basic knowledge about the mechanisms and biotechnological applications oflipid accumulation in yeasts.

Smart deep brain stimulation as a treatment strategy in treatment-resistant depression
Duration:	1. 1. 2022 - 31. 12. 2025
Evidence number:	2/0057/22
Program:	VEGA
Project leader:	MMedSc Dremencov Eliyahu, DrSc
SAS cosolvers:	MUDr. Grinchii Daniil, PhD., MUDr. Paliokha Ruslan, Ing. Mgr. Rosipal Roman, DrSc., Mgr. Rošťáková Zuzana, PhD.
Other cosolvers:	Ladis
Annotation:	Impaired connectivity between different brain areas underlines pathophysiology of multiple brain disorders. It is possible that impaired connectivity between prefrontal cortex and ventral pallidum is involved in depression. Smart deep brain simulation, combining real-time detection of the neuronal activity in in prefrontal cortex with the stimulation of ventral tegmental area, might be thus an effective in depression. We aim to examine the cortico-tegmental connectivity and to test the antidepressant-like effectiveness of the smart deep brain stimulation in an animal model of depression.

NANOREDUMET - Zinc feed additives as a strategy to reduce methane emissions in ruminants with endoparasites
Duration:	1. 1. 2024 - 31. 12. 2025
Evidence number:	SK-PL-23-0004
Program:	APVV
Project leader:	MVDr. Váradyová Zora, PhD
SAS cosolvers:	RNDr. Batťányi Dominika, PhD., Mgr. Bombárová Alexandra, RNDr. Čobanová Klaudia, PhD., Ing. Petrič Daniel, PhD.
Annotation:	Agriculture accounts for a significant share of all greenhouse gas emissions, according to the latest data, and reducing emissions is one of the biggest challenges in animal production. Most anthropogenic methane emissions come from enteric fermentation in ruminants. Reducing global methane emissions from livestock production should be linked to a general effort to combat the factors that influence climate change. Parasitic worms, the gastrointestinal nematodes of ruminants, affect several factors associated with methane emissions, including feed efficiency, nutrient utilization, and animal production. The proposed project design aims to investigate the anti-methanogenic effects of feed supplementation with zinc oxide nanoparticles on lambs with gastrointestinal nematodes.

Modulation of neuronal excitability by homocysteine
Duration:	1. 1. 2022 - 31. 12. 2025
Evidence number:	2/0081/22
Program:	VEGA
Project leader:	prof. RNDr. Lacinová Ľubica, DrSc.
SAS cosolvers:	Ing. Dubiel Lucia, PhD., Ing. Filipová Alžbeta, MUDr. Grinchii Daniil, PhD., Mgr. Jurkovičová Tarabová Bohumila, PhD., Mgr. Ševčíková Tomášková Zuzana, PhD., Mgr. Tomko Matúš, PhD.
Annotation:	Homocysteine (Hcy) is an intermediate of methionine metabolism physiologically presents in the plasma of healthy humans. Abnormally high plasma level of Hcy results in a metabolic condition called hyperhomocysteinemia (hhc). It is related to cardiovascular and neurological disorders including dementia, Parkinson’s disease, multiple sclerosis, epilepsy, and peripheral neuropathy. We will analyse the effect of hhc on the modulation of neuronal excitability at the level of single neurons and neuronal networks. Alteration of voltage-activated Na+, K+, and Ca2+ currents and ligand-gated glutamate and GABA receptors will be investigated in cultured primary thalamic neurons. The involvement of Ca2+ channel isoforms will be assessed in detail with the aim to clarify the underlying mechanisms. Finally, complex effects of changes in voltage- and ligand-gated channels on action potential firing will be characterized. The project will contribute to understand the mechanisms underlying neuropathologies related to hhc.

PostdokGrant - Non-traditional agricultural-industrial by-products as feed additives for sheep with endoparasites
Duration:	1. 7. 2024 - 31. 12. 2025
Evidence number:	APD0032
Program:	PostdokGrant
Project leader:	Ing. Petrič Daniel, PhD.
Annotation:	Parasitic gastrointestinal nematodes (GIN) in ruminants affect not only many factors related to the efficiency and useof feed but also contribute to the increased intensity of greenhouse gas emissions (GHG), especially methane. Theproject deals with the impact of locally available agro-industrial by-products, such as polyphenol-rich spent coffeegrounds used as feed additives in lambs with endoparasitosis. The aim of the project will be to what extent feedsupplementation affects rumen microbial fermentation, methanogenesis, and immunological parameters of lambsloaded with GIN. Obtaining new information about the potential use of these non-traditional agro-industrial byproductscould serve as an example of a circular economy with lower dependence on chemical inputs and better useof locally available sources of polyphenols in the agroecological context.
Project website:	https://postdokgrant.sav.sk/

PostdokGrant - Neurotrophin containing nanoparticles in the treatment of acute neuronal injury
Duration:	1. 7. 2024 - 31. 12. 2025
Evidence number:	ADP0092
Program:	PostdokGrant
Project leader:	RNDr. Ondáčová Katarína, PhD.
Annotation:	Acute damage to the central nervous system, such as traumatic brain injury (TBI), poses global medical and socioeconomic concerns. Challenges in its treatment arise due to limited neuroregeneration. TBI disrupts neural networks, impacting psychomotor functions. Successful tissue regeneration requires an adequate treatment of all associated pathological conditions. Any potential cure must pass the blood-brain barrier (BBB). We suggest an application of the brain-derived growth factor (BDNF) to the site of injury, as a candidate treatment, due to its role in promoting neuron survival and growth. To overcame a BBB we suggest use of novel polymeric nanoparticles as a drug carrier. Application of nanomedicine\'s role in treating neurodegenerative damage represents new promising path with a potential to enhance quality of life of TBI patients

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Duration:	1. 7. 2024 - 31. 12. 2025
Evidence number:	APD0096
Program:	PostdokGrant
Project leader:	Ing. Hamarová Ľudmila, PhD.

PostdokGrant - Monitoring the cytotoxic effect of two synthetic isothiocyanates, 1,4-chlorophenyl isothiocyanate and 1,4-chlorobenzyl isothiocyanate on human leukemia cells
Duration:	1. 7. 2024 - 31. 12. 2025
Evidence number:	APD0122
Program:	PostdokGrant
Project leader:	RNDr. Kšiňanová Martina, PhD.

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Duration:	1. 1. 2025 - 31. 12. 2025
Evidence number:	APP0595
Program:	DoktoGrant
Project leader:	MVDr. Zábolyová Natália

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Duration:	1. 1. 2025 - 31. 12. 2025
Evidence number:	APP0588
Program:	DoktoGrant
Project leader:	Mgr. Bombárová Alexandra
Annotation:	In recent years, zinc nanoparticles have been used with increasing frequency due to potentially greater zinc absorption and bioavailability than that observed for traditional inorganic forms. The presented project proposal isfocused on the effect of zinc nanoparticles as mineral supplements in animal diets on the antioxidant status as well as their adverse effect during long-term treatment. The impact of the zinc nanoparticles added to diets at doses up to the maximum EU authorised total Zn level for sheep, on the activity of antioxidant enzymes and oxidative stressindicators in plasma and tissues of lambs will be investigated. The inflammatory responses in serum and feces, histological changes in tissues, and animal performance will be monitored as well. The proposed project will providenew knowledge in the field of mineral nanoparticles used as feed additives in animal nutrition in order to improve thehealth and production of farm animals, but it also brings new questions related to their potential adverse effect in theorganism or the metabolic fate of nanoparticles in animal tissues.

PostdokGrant - The effect of combined therapy of cisplatin and isothiocyanates on the multidrug resistance phenotype in human leukemia cells
Duration:	1. 7. 2024 - 31. 12. 2025
Evidence number:	APD0124
Program:	PostdokGrant
Project leader:	Ing. Kontár Szilvia, PhD.
Annotation:	Chemotherapy is currently considered the most effective modality for the treatment of various types of cancer. Aserious obstacle to successful cancer treatment is the development of multidrug resistance (Multidrug Resistance,MDR) to a wide range of structurally and functionally different xenobiotics. MDR is often associated with increasedexpression of P-glycoprotein (P-gp), which eliminates chemotherapeutic agents from the intracellular to theextracellular space of the neoplastic cell, which usually leads to treatment failure or disease recurrence. The effort ofthe scientific community is the development of a safe strategy to overcome, or preventing P-gp-mediated MDR andimproving the survival rate of cancer patients. The use of isothiocyanates (ITCs), biologically active products ofglucosinolates, originating primarily from plants of the genus Brassicaceae, appears to be a promising option. Thelipophilic and electrophilic character of ITCs ensures their rapid absorption and high bioavailability, thanks to whichthey can apply their pharmacological benefits related to the anti-inflammatory, antioxidant, antimicrobial, cardio- andneuroprotective, as well as chemopreventive activity of these substances. The cytotoxic effect of higherconcentrations of aliphatic ITCs - sulforaphane (SFN) and allyl isothiocyanate (AITC) on mouse leukemia cells, whichleads to the induction of autophagy, respectively apoptosis, even if the cells showed an MDR phenotype, we alsoconfirmed in our laboratory. Current research shows to the possibility of using ITCs as a combined treatment withclinically used antineoplastic agents, which, in addition to reducing side effects and improving the effectiveness ofchemotherapeutics, can lead to overcoming chemoresistance. In the context of this idea, in the presented project Iwill focus on monitoring the possible use of two ITCs (aliphatic sulforaphane (SFN) and aromaticbenzylisothiocyanate (BITC)) as supporting agents in potentiating the cytotoxic effect of cisplatin (CisPt), to whichcells with an MDR phenotype based on overexpression of the efflux membrane pump P-gp show a lower sensitivity,although CisPt is not a substrate of this transporter.

The effect of UV-B radiation on DNA damage and repair mechanisms in the P-glycoprotein-expressing L1210 cell line
Duration:	1. 1. 2022 - 31. 12. 2025
Evidence number:	2/0141/22
Program:	VEGA
Project leader:	Mgr. Šereš Mário, PhD.
SAS cosolvers:	RNDr. Boháčová Viera, CSc., Ing. Kavcová Helena, RNDr. Kšiňanová Martina, PhD., Ing. Kyca Tomáš, PhD., Mgr. Pavlíková Lucia, PhD., Ing. Sulová Zdena, DrSc.

RNA structural determinant of Alus exonization
Duration:	1. 1. 2022 - 31. 12. 2025
Evidence number:	2/0016/22
Program:	VEGA
Project leader:	Mgr. Královičová Jana, PhD.
SAS cosolvers:	Ing. Borovská Ivana, PhD.

The role of phosphatidylglycerol in attenuation of inflammation
Duration:	1. 1. 2022 - 31. 12. 2025
Evidence number:	VEGA 2-0030-22
Program:	VEGA
Project leader:	Mgr. Balážová Mária, PhD.
Annotation:	In the context of current SARS-CoV-2 infections and accompanying pulmonary complications, attention needs to be focused on all aspects of the respiratory system. The virus is causing damage to type II alveolar cells, thus drastically reducing the production of lung surfactant lipids and proteins that are essential for respiration and their secretion into alveolar space. Understanding the defense mechanisms of the lungs plays a crucial role in responding to infection. One of the critical consequences of SARS-CoV-2 infection is the development of acute respiratory distress syndrome (ARDS). The main objective of this project is to contribute to the knowledge about the potential use of valproic acid to induce the synthesis of phosphatidylglycerol in the pulmonary surfactant. We hypothesize that intracellularly increased phosphatidylglycerol level could suppress inflammatory responses in our A549 alveolar tumor cell model and similarly in patients with ARDS.

Physiopathology of T-type calcium channels in motor neuron function
Duration:	1. 1. 2022 - 31. 12. 2025
Evidence number:	2/0073/22
Program:	VEGA
Project leader:	dr. Weiss Norbert, PhD.
SAS cosolvers:	Ing. Dubiel Lucia, PhD., Ing. Filipová Alžbeta, Mgr. Jurkovičová Tarabová Bohumila, PhD., prof. RNDr. Lacinová Ľubica, DrSc., Mgr. Tomko Matúš, PhD.

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Duration:	1. 7. 2024 - 31. 8. 2025
Evidence number:	09I03-03-V06-00024
Program:	Plán obnovy EÚ
Project leader:	MVDr. Fabian Dušan, DrSc.

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Duration:	1. 7. 2024 - 31. 8. 2025
Evidence number:	09I03-03-V06-00020
Program:	Plán obnovy EÚ
Project leader:	Mgr. Ševčíková Tomášková Zuzana, PhD.

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Duration:	1. 8. 2024 - 31. 7. 2025
Evidence number:	09I03-03-V06-00004
Program:	Plán obnovy EÚ
Project leader:	Mgr. Gaburjáková Marta, PhD.

Nano-Neuro-Plast - Activation of the VGF/BDNF/TrkB pathway by synthetic mRNA encapsulated in polyplex nanoparticles: effects on neural excitability, neuroplasticity and animal behavior
Duration:	1. 7. 2021 - 30. 6. 2025
Evidence number:	APVV-20-0202
Program:	APVV
Project leader:	MMedSc Dremencov Eliyahu, DrSc
SAS cosolvers:	Mgr. Cagala Martin, Mgr. Gaburjáková Jana, PhD., Mgr. Gaburjáková Marta, PhD., MUDr. Grinchii Daniil, PhD., prof. RNDr. Lacinová Ľubica, DrSc., Mgr. Macková Katarína, PhD., Mgr. Niederová-Kubíková Ľubica, PhD.
Annotation:	Neuroplasticity is the ability of the central nervous system (CNS) to change and adapt throughout the life through its structural and/or functional reorganization. Brain plasticity is essential for the normal CNS function, while several CNS disorders, such as depression and schizophrenia, are accompanied by decreased neuroplasticity. Neurotrophic growth factors, such as brain derived neurotrophic factor (BDNF), VGF, and fibroblast growth factor 2 (FGF2) play a key role in neuroplasticity. We will examine, for the first time, the effect of a non-invasive intra-CNS delivery of messenger RNA (mRNA) encoding these factors on neuronal excitability, plasticity, and animal behavior. Fluorescently labeled mRNA will be encapsulated in polyplex nanomicelles and administered intranasally. The incorporation of mRNA within the CNS will be assessed using STED microscopy. The effect of the exogeneous growth factor mRNA will be examined on neuronal excitability using in vitro and in vivo electrophysiology, on neurogenesis using the fluorescent immunohistochemical staining, on dendrito- and synaptogenesis using electron microscopy, on endogenous mRNA and protein expression-using the real-time PCR and Western blot analysis, and on intracellular calcium signaling using the method of planar lipid membranes. The effect of exogenous growth factor mRNA on the specific characteristic of animal behavior (cognition, social interacting, and anxiety) will be attested using appropriate behavioral tests. We expect that the polyplex nanomicelles to be designed will deliver mRNA encoding neurotrophic factors to the deep brain areas, with subsequent induction of pro-neuroplastic, pro-cognitive, anxiolytic and antidepressant-like effects. This non-invasive way of administration of a therapeutic mRNA into the brain might be used in the future as a next generation treatment strategy in depressive and psychotic disorders.

GLYCO4BIO - Biochip systems for targeted glycan analysis of biomarkers for biomedical and biotechnological applications
Duration:	1. 7. 2021 - 30. 6. 2025
Evidence number:	APVV-20-0243
Program:	APVV
Project leader:	Ing. Sulová Zdena, DrSc.
SAS cosolvers:	RNDr. Bertová Anna, PhD., RNDr. Imrichová Denisa, PhD., Ing. Kontár Szilvia, PhD.
Annotation:	The GLYCO4BIO project focuses on research and development of analytical systems based on microarraybiochips enabling targeted and high-throughput glycoprofiling and their use in biomedicine and biotechnology. Thisis a strongly interdisciplinary research with a high degree of innovation, especially in the field of construction andapplication of original biochip devices on the microarray platform. New systems for analyzing glycan structures invarious types of biological samples will be developed, verified and validated, such as a high-performancemicrofluidic reflectometric label-free microarray system, and an on-chip glycoprofiling platform combiningmicroarray and MS technology. The expected benefit of the presented project is mainly in the development ofinnovative biochip systems for targeted glycorecognition based on modern technologies and their use inbiomedicine, biotechnology, the study of biointeractions and in the analysis and screening of biomarkers. Thesystems will be applied, for example, in the research and detection of biomarkers of congenital disorders ofglycosylation (CDG), cancer, gestational diabetes, in oncological research, as well as in the development andcharacterization of the therapeutic proteins. The developed biochip systems significantly outperform traditionaltechniques and have a high potential for their translation into clinical analysis. The expected results of the projectwill improve and expand the possibilities of diagnostics and therapy, and significant benefits are also expected inthe expansion of knowledge in the field of biomedical research, glycoproteomics and biotechnology.

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Duration:	1. 7. 2024 - 30. 6. 2025
Evidence number:
Program:	Návratová projektová schéma
Project leader:	RNDr. Kopčáková Anna, PhD.

RENASTHERA - Novel renal antisense therapy platform for CKD
Duration:	1. 8. 2021 - 30. 6. 2025
Evidence number:	APVV-20-0494
Program:	APVV
Project leader:	Ing. Poturnayová Alexandra, PhD.
Annotation:	Chronic kidney disease (CKD) with its progressive nature towards end-stage renal disease (ESRD) is a lethal andrapidly progressing severe health complication associated with significantly decreased quality of life and highmortality rates. Strikingly, despite the progress made in early-diagnostics of CKD, state-of-the-art therapeutics donot significantly decrease the risk of renal and cardiovascular morbidity and mortality rates in CKD patients whichremain devastatingly high. This fact highlights an urgent need not only for novel therapeutics but also for theimplementation of progressive experimental and clinical tools into translational drug discovery. In this context, theproposed RENASTHERA project offers a novel therapeutic solution to stop progressive renal function loss. Thissolution is based on a patented method of nucleic acid inhibition. Periostin, a 90 kDa secreted protein wasidentified as a key player in CKD development, inhibition of which effectively prevented CKD progression. Thedesign, synthesis, and functional validation of an RNA inhibitor specifically designed for periostin RNA is thus theclearly defined scope of the proposed RENASTHERA project.

NONBOVQUAL - Evaluation method of raw goat milk microbiological quality and use of autochthonous lactic acid bacteria at non-bovine Milk processing
Duration:	1. 7. 2021 - 30. 6. 2025
Evidence number:	APVV-20-0204
Program:	APVV
Project leader:	MVDr. Lauková Andrea, CSc.
SAS cosolvers:	MVDr. Bino Eva, PharmDr. Focková Valentína, PhD., MVDr. Pogány Simonová Monika, PhD, RNDr. Ščerbová Jana, PhD.
Annotation:	The aim of the project submitted is to increase products quality made from non-bovine milks (ewes and goat). The value of total cell count in non bovine milk is their basic statutory indicator. In the past, a speedy routine method based on laser flow cytometry was introduced in Slovak practice for microbiolgical quality evaluation of raw ewes milk. This method proved to be beneficial for ewes milk producers and processors. However, for the evaluation of raw goat milk quality, solely the lenghty assigned cultivation method is still used. Therefore, the project aims to design a conversion formula specific for Slovakia to measure raw goat milk microbiological quality mean of laser flow cytometry method to CFU/ml which are used for statutory limits, and to the implementation of this method in practice as an accredited and authorized methods. For the improvement of dairy products quality based on non-bovine milks speedy methods which allow the separation of milk worth insufficient quality, do not constitute the only important condition. The other important condition consists in biological/bioprocessal methods of spoilage microbiota elimination during milk processing itself. Therefore, the subsequent aim of the project is the utilization of autochthonous beneficial, bacteriocin-producing lactic aicd bacteria strains with bacteriocin activity in products produced from non-bovine milks up to the implementation of such procedures in dairy processing.

IZOTIOVIVO - Molecular mechanisms of trialkyl-/triaryltin isothiocyanates\' and carboxylates\' antitumour properties - novel ligands of nuclear retinoid X receptors in rat mammary gland carcinomas and human tumour cell lines
Duration:	1. 7. 2021 - 30. 6. 2025
Evidence number:	APVV-20-0314
Program:	APVV
Project leader:	Mgr. Šereš Mário, PhD.
SAS cosolvers:	RNDr. Boháčová Viera, CSc., Mgr. Pavlíková Lucia, PhD., Mgr. Šereš Mário, PhD.
Annotation:	Organometallic compounds, on account of their structural diversity, are applied in human oncology in the treatmentof cancer. Triorganotin derivatives showed significant cytotoxic properties. At the cellular level, they induce massivecell death in various types of cell cultures even at low concentrations and are able to activate the processes ofapoptotic pathways, in which several molecular mechanisms play a role. A significant breakthrough in knowledgewas our recent experimental confirmation of the ability of molecules of triorganotin compounds to bind to nuclearretinoid X receptors, and thus function as potent agonists. The aim of the present project is to investigate the in vivo effects of tributyl/triphenyltin isothiocyanates in the process of chemical carcinogenesis of the mammary glandof female rats, which is based on our current results of in vitro experiments. Simultaneously, the research ofantitumour properties of triorganotin compounds activating RXR-RAR heterodimers comprising novel RXR agonistsbased on triorganotin carboxylates, is envisaged. In vitro analyses of molecular mechanisms leading to inhibition oftumour cell growth or induction of apoptosis in the presence/absence of natural ligands of RAR receptors onhuman breast tumour cell lines: MCF-7 (non-invasive, ER positive), T47D (ER positive), MDA-MB-231 (invasive,triple negative) and MDA-MB-436 (invasive, ER negative, PR negative), will be accomplished. We also plan toachieve new data on the possible endocrine disruption of triorganotin compounds on the murine TM3 cell line andthe human COV434 cell line representing the reproductive system. We assume that the presented project will gainnew and original knowledge about the mechanism of the action of the studied substances through their binding andactivation of nuclear receptors, their transactivation as well as crosslink with other signalling pathways that maycontribute to the development of novel treatment options for breast cancer.

HiVaLip - Nonconventional yeasts as producers of high value-added lipids
Duration:	1. 7. 2021 - 30. 6. 2025
Evidence number:	APVV-20-0166
Program:	APVV
Project leader:	Mgr. Holič Roman, PhD.
SAS cosolvers:	Mgr. Garaiová Martina, PhD., RNDr. Griač Peter, DrSc., RNDr. Hapala Ivan, CSc., Mgr. Krajčiová Daniela, Mgr. Petrisková Lívia, Soni Apoorva, Mgr. Valachovič Martin, PhD.
Other cosolvers:	pro
Annotation:	In addition to traditional baker`s yeast Saccharomyces cerevisiae there is a large group of „nonconventional“ yeasts which have evolved specific metabolic adaptations enabling them to utilize various growth substrates. An important group of nonconventional yeasts is represented by oleaginous yeasts which are able to accumulate high amount of lipids. Due to specific lipid metabolism and ability to utilize various cheap waste products as growth substrates, several nonconventional yeast species could be used for the production of high value-added lipids. The project is focused on the potential of nonconventional yeasts for the production of two such lipids – punicic acid and squalene. Punicic acid is a polyunsaturated fatty acid present in the pomegranate seed oil. Squalene is a triterpenoid produced in eukaryotes as the intermediate in sterol synthesis. Both lipids show positive effects on human health and have thus high potential for the use in cosmetic and pharmaceutical industries and as nutraceutics or additives to functional food and feedstuff. The project will focus on the effect of heterologous expression of genes from pomegranate on punicic acid production in the yeast Schizosaccharomyces pombe and in two oleaginous yeasts Rhodotorula (Rhodosporidium) toruloides and Yarrowia lipolytica. Accumulation of squalene will be studied by applying the methods of metabolic engineering in R. toruloides and Y. lipolytica. An important aim of the project is also lipid metabolism and production of lipids in selected species of the genus Magnusiomyces and screening of nonconventional yeasts from yeast collections for their potential to produce high value-added lipids. In the identified species, the ability to utilize cheap waste substrates for growth will be determined. The results obtained within project implementation could thus extend the use of nonconventional yeasts in the biotechnological production of commercially valuable lipids.

LIPinINF - The potential role of valproic acid in attenuation of inflammation
Duration:	1. 8. 2021 - 30. 6. 2025
Evidence number:	APVV-20-0129
Program:	APVV
Project leader:	Mgr. Balážová Mária, PhD.
Other cosolvers:	Prírodovedeck
Annotation:	In the context of current SARS-CoV-2 infections and accompanying pulmonary complications, attention needs to be focused on all aspects of the respiratory system. The virus is causing damage to type II alveolar cells, thusdrastically reducing the production of lung surfactant lipids and proteins that are essential for respiration and theirsecretion into alveolar space. Understanding the defense mechanisms of the lungs plays a crucial role in responding to infection. One of the critical consequences of SARS-CoV-2 infection is the development of acute respiratory distress syndrome (ARDS).The main objective of this project is to contribute to the knowledge about the potential use of valproic acid to induce the synthesis of phosphatidylglycerol in the pulmonary surfactant. To answer specific questions, we will use three models differing in their complexity – yeast (Saccharomyces cerevisiae), human tumor cell line (HAP1), and avian chorioallantoic membrane. We hypothesize that intracellularly increased phosphatidylglycerol level could suppress inflammatory responses in ARDS patients and reduce the attachment of the virus to cell surface receptors. This would mean a reduction in infectious particle transmission between individuals and significant alleviation of ARDS symptoms.

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Duration:	24. 5. 2024 - 30. 6. 2025
Evidence number:
Program:	Iné projekty
Project leader:	RNDr. Bíliková Katarína, PhD.

Optogenetika u s - Examining the role of dopamine and adult neurogenesis in learned behavior of songbirds using optogenetic manipulation
Duration:	1. 7. 2021 - 30. 6. 2025
Evidence number:	APVV-20-0344
Program:	APVV
Project leader:	Mgr. Niederová-Kubíková Ľubica, PhD.
SAS cosolvers:	RNDr. Bilčík Boris, PhD., Mgr. Buraľová Martina, Mgr. Hoďová Vladimíra, MUDr. Mgr. Hromádka Tomáš, PhD., RNDr. Košťál Ľubor, CSc., Mgr. Máčajová Mariana, PhD., Mgr. Pavuková Eva, PhD.
Annotation:	Dopamine signaling and neurogenesis play an important role in structural and functional neuroplasticity in the adultbrain. In this project, we focus on the role of the dopaminergic neurotransmission and the role of newborn neuronsfor the control of learned motor sequences in songbirds. While there are numerous studies about motor sequencelearning with external reward such as food, studies that involve internal reward system are rare. The internalreward system has been linked with dopamine signalization, and the songbird vocal learning and productionrepresent a great model for such study since the birdsong comprises sequences of very fast actions. We proposeto use the modern technique of optogenetics to manipulate vocal sequences. The optogenetic approach enablesprecise control of neural activity in a specific brain region in a freely moving animal with millisecond precision. Herewe propose to modify dopamine reward signaling to the striatal part of basal ganglia and examine the changes inthe song sequence. The results will determine the role of dopamine reward system in birdsong learning. In thesecond part of the project, we propose to optogenetically manipulate the activity of new neurons incorporated to thebrain region necessary for song production. The number of newborn neurons in this region increases at the timewhen the song structure is more variable and new syllables are integrated to the song. We will find in the projectwhether new neurons born in adulthood are able to modify the song and whether they enable or contribute to thesong variability.

The effect of punicic acid on fertilization and preimplantation embryo development in obesity
Duration:	1. 7. 2024 - 30. 6. 2025
Evidence number:
Program:	Návratová projektová schéma
Project leader:	RNDr. Babeľová Janka, PhD.
Annotation:	The nutritional status of the mother during pregnancy is an important environmental factor that can affect gene expression and the developmental potential of the embryo and fetus. The interactions of several internal and external factors cause the accumulation of undesirable amounts of free radicals, which leads to oxidative stress and the creation of an unsuitable environment for normal reproduction. Enriching diets with beneficial lipids could be a suitable strategy to reduce the frequency of reproductive failure. Research has shown that polyunsaturated fatty acids (PUFAs) are extremely important and almost essential during pregnancy. The main goal of the project will be to evaluate the effect of enriching the diet with punic acid on the success of fertilization and to reduce early embryonic failure in an animal model – female mice. The obtained results will be important for human reproductive medicine.